Pham Thi Phuong1*, Nguyen Vu Phong2 , Pham Duc Toan3 , Hoang Manh Cuong4
1 Tay Nguyen University
2 Ho Chi Minh City University of Agriculture and Forestry
3 Research Institute for Biotechnology and Enviroment, Ho Chi Minh City University of Agriculture and Forestry.
4 Western Highland Agriculture and Forestry Science Institute (WASI)
SUMMARY
The thesis’ main purpose is assessment of genetic diversity of twenty-four avocado (Persea americana Mill.) varieties in Western Highland Agriculture and Forestry Science Institute (WASI) based on morphological characteristics and molecular marker SSR (Simple Sequence Repeat). Based on morphological characteristics, twenty-four avocado varieties grouped into three clusters including fourteen, six, four genotypes, respectively. The PCR analysis with 18 molecular markers SSR obtained 59 polymorphism bands of 62 amplified bands with sizes ranging from 70 bp to 350 bp. There is an average of 3,44 DNA band per marker. The general ratio of polymorphism bands in the marker is ninety-six per cent. Three markers have five polymorphism bands are AVAG13, AVAG21 and LMAV.33. The polymorphic information content (PIC) ranged from 0,12 to 0,90 for all markers, in which LMAV.06 and LMAV.33 were the highest polymorphism marker. The results also indicated that the similarity coefficient of twenty-four avocado varieties varied from 0,12 to 0,99. The phylogenetic analysis divided the genotypes into six main groups as similarity coefficient by 0,394. Finally, phenotype and genotype data were combined to provide an initial knowledge about the race of avocado varieties in our country.
Keywords: Avocado, genetic diversity, SSR.