{"id":1251,"date":"2016-07-11T08:52:45","date_gmt":"2016-07-11T01:52:45","guid":{"rendered":"http:\/\/wasi.org.vn\/vi\/?p=1251"},"modified":"2017-12-27T10:39:04","modified_gmt":"2017-12-27T03:39:04","slug":"quy-trinh-san-xuat-che-pham-sinh-hoc-xu-ly-vo-ca-phe-o-quy-mo-nho","status":"publish","type":"post","link":"https:\/\/wasi.org.vn\/en\/quy-trinh-san-xuat-che-pham-sinh-hoc-xu-ly-vo-ca-phe-o-quy-mo-nho\/","title":{"rendered":"Quy tr\u00ecnh s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m sinh h\u1ecdc x\u1eed l\u00fd v\u1ecf c\u00e0 ph\u00ea \u1edf quy m\u00f4 nh\u1ecf"},"content":{"rendered":"

GI\u01a0\u0301I THI\u00ca\u0323U CHUNG<\/strong><\/span><\/p>\n

\u00a0<\/strong><\/span><\/p>\n

Quy tri\u0300nh na\u0300y la\u0300 k\u00ea\u0301t qua\u0309 nghi\u00ean c\u01b0\u0301u cu\u0309a \u0111\u00ea\u0300 ta\u0300i c\u00e2\u0301p c\u01a1 s\u01a1\u0309: \u201cNghi\u00ean c\u1ee9u s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m sinh h\u1ecdc s\u1eed d\u1ee5ng trong x\u1eed l\u00fd v\u1ecf qu\u1ea3 c\u00e0 ph\u00ea l\u00e0m ph\u00e2n b\u00f3n cho c\u00e2y tr\u1ed3ng<\/em>“<\/em> do Vi\u00ea\u0323n Khoa ho\u0323c Ky\u0303 thu\u00e2\u0323t N\u00f4ng L\u00e2m nghi\u00ea\u0323p T\u00e2y Nguy\u00ean chu\u0309 tri\u0300. \u0110\u00ea\u0300 ta\u0300i na\u0300y \u0111\u01b0\u01a1\u0323c th\u01b0\u0323c hi\u00ea\u0323n t\u01b0\u0300 tha\u0301ng 1 n\u0103m 2006 \u0111\u00ea\u0301n tha\u0301ng 12 n\u0103m 2010.<\/span><\/p>\n

T\u00ean quy tri\u0300nh: Quy tr\u00ecnh s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m sinh h\u1ecdc x\u1eed l\u00fd v\u1ecf c\u00e0 ph\u00ea \u1edf quy m\u00f4 nh\u1ecf<\/span><\/p>\n

T\u00ean \u0111\u01a1n vi\u0323 soa\u0323n tha\u0309o: Vi\u00ea\u0323n Khoa ho\u0323c Ky\u0303 thu\u00e2\u0323t N\u00f4ng L\u00e2m nghi\u00ea\u0323p T\u00e2y Nguy\u00ean – Vi\u00ea\u0323n Khoa ho\u0323c N\u00f4ng nghi\u00ea\u0323p Vi\u00ea\u0323t Nam<\/span><\/p>\n

Ca\u0301c ta\u0301c gia\u0309: \u0110a\u0300o Thi\u0323 Lan Hoa, Cu\u0300 Thi\u0323 D\u00e2\u0300n, Nguy\u00ea\u0303n Thi\u0323 V\u00e2n, Nguy\u00ea\u0303n Thi\u0323 Thi\u00ean Trang, Nguy\u00ea\u0303n Thi\u0323 Ti\u00ea\u0301n Sy\u0303, Nguy\u00ea\u0303n Xu\u00e2n Ho\u0300a, Vo\u0303 Thi\u0323 Kim Oanh, va\u0300 c\u00f4\u0323ng s\u01b0\u0323.<\/span><\/p>\n

T\u00ean c\u01a1 quan ban ha\u0300nh: Vi\u00ea\u0323n Khoa ho\u0323c Ky\u0303 thu\u00e2\u0323t N\u00f4ng L\u00e2m nghi\u00ea\u0323p T\u00e2y Nguy\u00ean<\/span><\/p>\n


\n <\/strong><\/span><\/p>\n

QUY TRI\u0300NH SA\u0309N XU\u00c2\u0301T CH\u00ca\u0301 PH\u00c2\u0309M SINH HO\u0323C \u00a0<\/strong><\/span>X\u01af\u0309 LY\u0301 VO\u0309 CA\u0300 PH\u00ca \u01a0\u0309 QUY M\u00d4 NHO\u0309<\/strong><\/span><\/p>\n

\u00a0<\/strong><\/span><\/p>\n

    \n
  1. PH\u00c2\u0300N QUY \u0110I\u0323NH CHUNG<\/strong><\/span><\/li>\n<\/ol>\n

    1.1. Pha\u0323m vi va\u0300 \u0111\u00f4\u0301i t\u01b0\u01a1\u0323ng a\u0301p du\u0323ng<\/strong><\/span><\/p>\n

    Quy tr\u00ecnh n\u00e0y \u0111\u01b0\u01a1\u0323c a\u0301p du\u0323ng \u0111\u00ea\u0309 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m sinh h\u1ecdc x\u1eed l\u00fd vo\u0309 ca\u0300 ph\u00ea \u1edf quy m\u00f4 nh\u1ecf t\u1ea1i c\u00e1c c\u01a1 s\u1edf nghi\u00ean c\u1ee9u v\u00e0 chuy\u1ec3n giao k\u1ef9 thu\u1eadt.<\/span><\/p>\n

    1.2. C\u01a1 s\u01a1\u0309 x\u00e2y d\u01b0\u0323ng quy tri\u0300nh<\/strong><\/span><\/p>\n

    \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Quy tr\u00ecnh \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng, \u0111\u00fac k\u1ebft d\u1ef1a tr\u00ean k\u1ebft qu\u1ea3 c\u1ee7a \u0111\u1ec1 t\u00e0i v\u00e0 tham kha\u0309o ta\u0300i li\u00ea\u0323u v\u00ea\u0300 sa\u0309n xu\u00e2\u0301t ch\u00ea\u0301 ph\u00e2\u0309m sinh ho\u0323c.<\/span><\/p>\n

    1.3. Mu\u0323c ti\u00eau kinh t\u00ea\u0301 ky\u0303 thu\u00e2\u0323t<\/strong><\/span><\/p>\n

    Quy tri\u0300nh d\u00ea\u0303 a\u0301p du\u0323ng, co\u0301 kha\u0309 n\u0103ng \u00e1p d\u1ee5ng \u1edf c\u00e1c \u0111\u01a1n v\u1ecb c\u00f3 \u0111\u1ea7y \u0111\u1ee7 \u0111i\u1ec1u ki\u1ec7n v\u1ec1 nh\u00e2n l\u1ef1c v\u00e0 trang thi\u1ebft b\u1ecb nghi\u00ean c\u1ee9u vi sinh v\u1eadt.<\/span><\/p>\n

      \n
    1. PH\u00c2\u0300N QUY \u0110I\u0323NH V\u00ca\u0300 KY\u0303 THU\u00c2\u0323T<\/strong><\/span><\/li>\n<\/ol>\n

      2.1.\u00a0 Nguy\u00ean v\u1eadt li\u1ec7u<\/strong><\/span><\/p>\n

      – C\u00e1c ch\u1ee7ng vi sinh v\u1eadt \u0111\u01b0\u1ee3c WASI ch\u1ecdn l\u1ecdc:<\/span><\/p>\n

      + 6 k\u00fd hi\u1ec7u m\u1eabu x\u1ea1 khu\u1ea9n (Streptomyces<\/em> spp.): SPC13, SPC29, SPC41, SPC72, SPC82, SVC18<\/span><\/p>\n

      + 5 k\u00fd hi\u1ec7u m\u1eabu n\u1ea5m (Aspergillus spp.)<\/em>: NP7, NV4, NV5, NV6, NV19<\/span><\/p>\n

      + 4 k\u00fd hi\u1ec7u m\u1eabu vi khu\u1ea9n (Bacillus spp.)<\/em>: VP8, VV18, VV26, VV36<\/span><\/p>\n

      2.2. Trang thi\u1ebft b\u1ecb, d\u1ee5ng c\u1ee5<\/strong><\/span><\/p>\n

      \u00a0<\/strong>– Trang thi\u1ebft b\u1ecb ph\u1ee5c v\u1ee5 nghi\u00ean c\u1ee9u vi sinh v\u1eadt: n\u1ed3i h\u1ea5p, t\u1ee7 c\u1ea5y, t\u1ee7 \u0111\u1ecbnh \u00f4n, t\u1ee7 b\u1ea3o qu\u1ea3n m\u1eabu, k\u00ednh hi\u1ec3n vi, c\u00e2n k\u1ef9 thu\u1eadt, m\u00e1y \u0111o pH, m\u00e1y l\u1eafc…<\/span><\/p>\n

      \u00a0– D\u1ee5ng c\u1ee5: \u0111\u0129a petri, \u1ed1ng nghi\u1ec7m, b\u00ecnh tam gi\u00e1c, chai th\u1ee7y tinh duran, que c\u1ea5y, dao scapel, pipet \u0111i\u1ec7n t\u1eed, th\u00f9ng g\u1ed7, b\u1ea1t<\/span><\/p>\n

      2.3. C\u00e1ch ti\u1ebfn h\u00e0nh<\/strong><\/span><\/p>\n

      T\u1eeb c\u00e1c ch\u1ee7ng vi sinh v\u1eadt g\u1ed1c \u0111\u01b0\u1ee3c ch\u1ecdn l\u1ecdc, bao g\u1ed3m: 6 k\u00fd hi\u1ec7u m\u1eabu x\u1ea1 \u00a0khu\u1ea9n, 5 k\u00fd hi\u1ec7u m\u1eabu n\u1ea5m m\u1ed1c, 4 k\u00fd hi\u1ec7u m\u1eabu vi khu\u1ea9n. \u0110\u00e2y l\u00e0 c\u00e1c m\u1eabu c\u00f3 \u0111\u1eb7c t\u00ednh sinh h\u1ecdc t\u1ed1t: C\u00e1c k\u00fd hi\u1ec7u x\u1ea1 khu\u1ea9n c\u00f3 ho\u1ea1t \u0111\u1ed9 enzyme cao, c\u00f3 kh\u1ea3 n\u0103ng sinh tr\u01b0\u1edfng t\u1ed1t, ch\u1ecbu \u0111\u01b0\u1ee3c nhi\u1ec7t \u0111\u1ed9 50 o<\/sup>C, th\u00edch \u1ee9ng pH 4 – 7; C\u00e1c k\u00fd hi\u1ec7u m\u1eabu n\u1ea5m v\u00e0 vi khu\u1ea9n c\u00f3 ho\u1ea1t t\u00ednh cellulase cao, c\u00f3 kh\u1ea3 n\u0103ng ch\u1ecbu nhi\u1ec7t \u0111\u1ed9 70 o<\/sup>C v\u00e0 th\u00edch \u1ee9ng pH\u00a0 4 – 7.<\/span><\/p>\n

      Ti\u1ebfn h\u00e0nh nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng vi sinh v\u1eadt g\u1ed1c tr\u00ean m\u00f4i tr\u01b0\u1eddng \u0111\u1eb7c chuy\u00ean t\u00ednh t\u01b0\u01a1ng \u1ee9ng tr\u00ean \u0111\u0129a petri. X\u1ea1 khu\u1ea9n \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng x\u1ea1 khu\u1ea9n trong th\u1eddi gian t\u1eeb 10 – 15 ng\u00e0y. N\u1ea5m m\u1ed1c \u0111\u01b0\u1ee3c\u00a0 nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng PDA t\u1eeb 5 – 7 ng\u00e0y. Vi khu\u1ea9n \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng vi khu\u1ea9n t\u1eeb 5 – 7 ng\u00e0y.<\/span><\/p>\n

      Ti\u1ebfn h\u00e0nh nh\u00e2n sinh kh\u1ed1i l\u1ecfng ri\u00eang bi\u1ec7t c\u00e1c ch\u1ee7ng x\u1ea1 khu\u1ea9n, n\u1ea5m m\u1ed1c v\u00e0 vi khu\u1ea9n tr\u00ean c\u00e1c m\u00f4i tr\u01b0\u1eddng t\u01b0\u01a1ng \u1ee9ng v\u1edbi kho\u1ea3ng th\u1eddi gian 3 ng\u00e0y. Sau \u0111\u00f3 c\u00e1c ch\u1ee7ng n\u00e0y ti\u1ebfp t\u1ee5c \u0111\u01b0\u1ee3c nh\u00e2n sinh kh\u1ed1i tr\u00ean m\u00f4i tr\u01b0\u1eddng l\u1ecfng h\u1ed7n h\u1ee3p trong th\u1eddi gian 3 – 5 ng\u00e0y. H\u1ed7n h\u1ee3p d\u1ecbch nu\u00f4i c\u1ea5y s\u1ebd \u0111\u01b0\u1ee3c tr\u1ed9n v\u00e0o c\u01a1 ch\u1ea5t (b\u1eafp c\u00e1m tr\u1ea5u) \u0111\u00e3 \u0111\u01b0\u1ee3c h\u1ea5p kh\u1eed tr\u00f9ng v\u00e0 \u1ee7 trong v\u00f2ng 5 ng\u00e0y. Trong th\u1eddi gian n\u00e0y c\u1ea7n ti\u1ebfn h\u00e0nh \u0111\u1ea3o tr\u1ed9n c\u01a1 ch\u1ea5t h\u00e0ng ng\u00e0y. Ch\u1ea5t ph\u1ee5 gia \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng l\u00e0 than b\u00f9n \u0111\u00e3 \u0111\u01b0\u1ee3c h\u1ea5p kh\u1eed tr\u00f9ng, s\u1ebd \u0111\u01b0\u1ee3c tr\u1ed9n v\u00e0o h\u1ed7n h\u1ee3p c\u01a1 ch\u1ea5t v\u00e0 \u1ee7 1 ng\u00e0y. Sau \u0111\u00f3 ti\u1ebfn h\u00e0nh ph\u01a1i, \u0111\u1ea3o cho kh\u00f4 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng. Khi ch\u1ebf ph\u1ea9m kh\u00f4, ti\u1ebfn h\u00e0nh ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 \u0111\u00f3ng g\u00f3i, b\u1ea3o qu\u1ea3n v\u00e0 s\u1eed d\u1ee5ng \u0111\u1ec3 x\u1eed l\u00fd v\u1ecf c\u00e0 ph\u00ea l\u00e0m ph\u00e2n b\u00f3n cho c\u00e2y tr\u1ed3ng.<\/span><\/p>\n

      \"cafe1\"<\/a> \"cafe2\"<\/a><\/p>\n

      S\u01a1 \u0111\u1ed3 quy tr\u00ecnh s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m sinh h\u1ecdc x\u1eed l\u00fd v\u1ecf c\u00e0 ph\u00ea \u1edf quy m\u00f4 nh\u1ecf<\/strong><\/span><\/p>\n

      \u00a0<\/p>\n

      \u00a0<\/p>\n