(Tiếng Việt) Kết quả xây dựng mô hình tích hợp các giải pháp tái canh cà phê ngay trên nền đất không luân canh tại Đắk Lắk(18/07/2018)

Abstract Coffee replanting is considered as one of the most important programs for sustainable coffee development in Vietnam. The results of coffee replanting depend on coffee garden condition and applied replanting techniques. In order to contribute to the success of this program, our study used all technical solutions on replanting coffee was conducted from 2016 to 2017 in Dak Lak province. The results showed that percentage of died trees were…

Ralstonia solanacearum (Smith) is an important pathogen worldwide. A new pair of primers, UDP-F and UDP-R, was designed based on the 159 bp target fragment of Chen et al. (2010) which is originated from the sequence of the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene conserved only in R. solanacearum. The result indicated that this pair of primers could be used to detect R. solanacearum directly from the bacterial suspensions by using conventional polymerase chain reaction (PCR) technique. However, these primers had so far only been tested with close relatives (Pseudomonas syzygii and the blood disease bacterium (BDB)) of R. solanacearum, so further experimentation should be carried out to optimize the PCR technique and check the specificity and sensitivity of primers and the protocol. Furthermore, the application of these primers in real time PCR should be investigated.